Imaging Extensions:
Densitometry Tools


The Densitometry Tools are part of the BIOQUANT Imaging Extensions add-on. They provide tools for receptor binding assays, quantitative autoradiography, in-situ hybridization histochemistry, and whole-body autoradiography. They also add support for grayscale digital cameras and images.

Selected Software Features

  • Curve fitting for Density Calibrations using seven types of curves including exponential, linear, logarithmic, power, and N-degree polynomial.
  • Calibration curve shows R2 for the curve and relative error for each standard value
  • Data, histograms, and pseudocolor palettes reported in calibrated units
  • Pseudocolor palettes automatically generated from standards
  • Easily save pseudocolor images with overlaid pseudocolor calibration bars
  • Place, move, and erase sampling areas in preview mode before saving data
  • Automatic mode records data for objects that meet threshold and geometric filter criteria

Representative Applications

  • Receptor Binding Assays
  • Quantitative Autoradiography
  • In-situ Hybridization Histochemistry
  • Whole-body Autoradiography
  • Genome Size Quantification by Feulgen Staining

Main Supported Hardware

  • Use Fuji BAS 5000 Phosphorimager to generate IMG + INF files that can be opened in BIOQUANT.
  • Capture direct, live monochrome images using the QImaging RETIGA EXI Monochrome Camera.
  • Digitize film with high-resolution, 4+ OD scanner to generate a 16-bit high resolution TIFF files that can be opened in BIOQUANT.

Download the Brochure

See the BIOQUANT Imaging Extensions Add-on Brochure.