Axon Number and Caliber

Toluidine Blue Imaging

Partial Nerve Montage using TolBlue. The greater the contrast the more automated your protocol will be.

In Glaucoma and Neuropathy Models

In semi-thin sections with myelin specific staining, BIOQUANT automatically detects myelinated axons. Detection is based on color, shape, and size. Shape filters automatically exclude obliquely cut axons. 

In the case of very small axons or partially myelnated axons, some manual editing may be necessary.

A unique object recognition algorithm prevents the system from double-counting when scanning through adjacent overlapping fields of view. 

Large scale projects have counted 50% of the axons in a non-human primate optic nerve.

Define and Collect Nerve Data

Define the nerve borders using the Irregular Region of Interest tool. BIOQUANT will limit threshold to within the ROI and retrieve Nerve Area data.

Computed Data

  • Nerve Area
  • Individual Axon Area
  • Individual Axon Caliber
  • Axon Count
  • Axon Density (#/unit area)
  • Mean Axon Area
  • Mean Axon Caliber